mouse anti human clec10a cd301 conjugated (R&D Systems)
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Mouse Anti Human Clec10a Cd301 Conjugated, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd301/Human+CLEC10A%2FCD301+Antibody/pm41525833-64-53-62
Average 92 stars, based on 7 article reviews
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Flow Cytometry:Article Title: CCR2 − and CCR2 + corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion Article Snippet: The corneal tissues were cut into pieces and digested with 0.2% collagenase type I (Sigma-Aldrich; no. C0130) for 1.5–2 h. The digested cells were washed two times with PBS and passed through a 75 μm filter to obtain single cells. .. These single cells were blocked in Flow Cytometry Staining Buffer (eBioscience; no. 00-4222) containing anti-mouse CD16/32 antibody (eBioscience; no. 14-0161-85) at room temperature for 10 min, followed by incubation at room temperature for 30 min with a mixture of the following antibodies (dilution 1:100): anti-mouse CD45 antibody conjugated with FITC (BD Biosciences, San Jose, CA; no. 553080), anti-mouse CD64 conjugated with Brilliant violet 421 (BioLegend; no. 139309) (anti-mouse CCR2 (R&D Systems; no. FAB5538A) or CX3CR1 (BioLegend; no. 149007) or CD206 (BioLegend; no. 141707) conjugated with allophycocyanin), anti-mouse CD11b conjugated with Percp Cy5.5 (eBioscience; no. 45-0112-82) (anti-mouse F4/80 (eBioscience; no. 12-4801-82) or Cytometry:Article Title: CCR2 − and CCR2 + corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion Article Snippet: The corneal tissues were cut into pieces and digested with 0.2% collagenase type I (Sigma-Aldrich; no. C0130) for 1.5–2 h. The digested cells were washed two times with PBS and passed through a 75 μm filter to obtain single cells. .. These single cells were blocked in Flow Cytometry Staining Buffer (eBioscience; no. 00-4222) containing anti-mouse CD16/32 antibody (eBioscience; no. 14-0161-85) at room temperature for 10 min, followed by incubation at room temperature for 30 min with a mixture of the following antibodies (dilution 1:100): anti-mouse CD45 antibody conjugated with FITC (BD Biosciences, San Jose, CA; no. 553080), anti-mouse CD64 conjugated with Brilliant violet 421 (BioLegend; no. 139309) (anti-mouse CCR2 (R&D Systems; no. FAB5538A) or CX3CR1 (BioLegend; no. 149007) or CD206 (BioLegend; no. 141707) conjugated with allophycocyanin), anti-mouse CD11b conjugated with Percp Cy5.5 (eBioscience; no. 45-0112-82) (anti-mouse F4/80 (eBioscience; no. 12-4801-82) or Staining:Article Title: CCR2 − and CCR2 + corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion Article Snippet: The corneal tissues were cut into pieces and digested with 0.2% collagenase type I (Sigma-Aldrich; no. C0130) for 1.5–2 h. The digested cells were washed two times with PBS and passed through a 75 μm filter to obtain single cells. .. These single cells were blocked in Flow Cytometry Staining Buffer (eBioscience; no. 00-4222) containing anti-mouse CD16/32 antibody (eBioscience; no. 14-0161-85) at room temperature for 10 min, followed by incubation at room temperature for 30 min with a mixture of the following antibodies (dilution 1:100): anti-mouse CD45 antibody conjugated with FITC (BD Biosciences, San Jose, CA; no. 553080), anti-mouse CD64 conjugated with Brilliant violet 421 (BioLegend; no. 139309) (anti-mouse CCR2 (R&D Systems; no. FAB5538A) or CX3CR1 (BioLegend; no. 149007) or CD206 (BioLegend; no. 141707) conjugated with allophycocyanin), anti-mouse CD11b conjugated with Percp Cy5.5 (eBioscience; no. 45-0112-82) (anti-mouse F4/80 (eBioscience; no. 12-4801-82) or Incubation:Article Title: CCR2 − and CCR2 + corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion Article Snippet: The corneal tissues were cut into pieces and digested with 0.2% collagenase type I (Sigma-Aldrich; no. C0130) for 1.5–2 h. The digested cells were washed two times with PBS and passed through a 75 μm filter to obtain single cells. .. These single cells were blocked in Flow Cytometry Staining Buffer (eBioscience; no. 00-4222) containing anti-mouse CD16/32 antibody (eBioscience; no. 14-0161-85) at room temperature for 10 min, followed by incubation at room temperature for 30 min with a mixture of the following antibodies (dilution 1:100): anti-mouse CD45 antibody conjugated with FITC (BD Biosciences, San Jose, CA; no. 553080), anti-mouse CD64 conjugated with Brilliant violet 421 (BioLegend; no. 139309) (anti-mouse CCR2 (R&D Systems; no. FAB5538A) or CX3CR1 (BioLegend; no. 149007) or CD206 (BioLegend; no. 141707) conjugated with allophycocyanin), anti-mouse CD11b conjugated with Percp Cy5.5 (eBioscience; no. 45-0112-82) (anti-mouse F4/80 (eBioscience; no. 12-4801-82) or Immunopeptidomics:Article Title: CCR2 − and CCR2 + corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion Article Snippet: The corneal tissues were cut into pieces and digested with 0.2% collagenase type I (Sigma-Aldrich; no. C0130) for 1.5–2 h. The digested cells were washed two times with PBS and passed through a 75 μm filter to obtain single cells. .. These single cells were blocked in Flow Cytometry Staining Buffer (eBioscience; no. 00-4222) containing anti-mouse CD16/32 antibody (eBioscience; no. 14-0161-85) at room temperature for 10 min, followed by incubation at room temperature for 30 min with a mixture of the following antibodies (dilution 1:100): anti-mouse CD45 antibody conjugated with FITC (BD Biosciences, San Jose, CA; no. 553080), anti-mouse CD64 conjugated with Brilliant violet 421 (BioLegend; no. 139309) (anti-mouse CCR2 (R&D Systems; no. FAB5538A) or CX3CR1 (BioLegend; no. 149007) or CD206 (BioLegend; no. 141707) conjugated with allophycocyanin), anti-mouse CD11b conjugated with Percp Cy5.5 (eBioscience; no. 45-0112-82) (anti-mouse F4/80 (eBioscience; no. 12-4801-82) or |
